Andrew P. Butler, Ph.D.
1977, University of Oregon
UT M.D. Anderson Cancer Center
Science Park/Research Division
My laboratory is located in Smithville, Texas, at UT Science Park -- Research Division.
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Andrew P. Butler, Ph.D. 1977, University of Oregon UT M.D. Anderson Cancer Center My laboratory is located in Smithville, Texas, at UT Science Park -- Research Division. |
Research Interests: Transcription, cell cycle, signal transduction, oncogenes, tumor promotion, skin cancer
My lab is studying the transcriptional regulation of genes involved in growth regulation of cancer cells. A major theme is the involvement of oncogenes in signal transduction and as transcription factors. These studies use cell culture and animal models, as well as molecular and biochemical assays of DNA-protein interactions and gene transcription. Current projects include transcriptional regulation of cyclin-dependent kinase inhibitors in a Xiphophorus fish melanoma model, and regulation of FoxO transcription factors. For details, see our lab home page: http://sciencepark.mdanderson.org/labs/butler/
A tutorial in my lab would provide experience in a variety of approaches for study of transcriptional regulation in cancer cells, including luciferase reporter gene assays, site-directed mutagenesis, RT-PCR, sequence analysis, Western and Northern blotting.
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Liu, J-W, Chandra, D, Rudd, MD, Butler, AP, Pallotta, V, Brown, D, Coffer, PJ, Tang, DG “Induction of pro-survival molecules by apoptotic stimuli: Involvement of FOXO3a and ROS and implications for cancer therapy,” Oncogene 24:2020-2031 (2005).
Rudd M.D., Johnston D. A., Kazianis S., and Butler, A.P. “Cloning and analysis of a FoxO transcription factor from Xiphophorus,” Gene 302:31-41 (2003).
Nairn R.S., Kazianis S., Della Coletta L., Trono D., Butler A. P., Walter R. B., and Morizot D. C. "Genetic analysis of susceptibility to spontaneous and UV carcinogenesis in Xiphophorus hybrid fish" Marine Biotech. 3:S24-S36 (2001).
Zhao, B., A. P. Kumar and A. P. Butler (2000) "A negative regulatory element within the proximal promoter region of the rat ornithine decarboxylase gene," Molec. Carcinogenesis29:212-218.
Kumar AP and Butler AP (1997) Transcription factor Sp3 antagonizes activation of the ornithine decarboxylase promoter by Sp1. Nucl Acids Res, 25: 2012-2019.
Program Affiliation:
Program in Molecular
Carcinogenesis