William P. Dubinsky, Ph.D. 1976, St. Louis University UT-Houston Medical School Integrative Biology and Pharmacology Contact Information Biographical Sketch

Research Interests: Membrane physiology; ion transport; reconstitution; regulation of ion fluxes; cystic fibrosis; epithelia

The long range objective of my research is to identify factors controlling membrane permeabilities at the molecular level. The model system currently under study is bovine tracheal epithelial Cl and Na transport. The approach involves detergent solubilization of purified membranes to extract transport complexes. These complexes are resolved into phospholipid vesicles. The studies are being extended to human tissues from normal and cystic fibrosis patients in an effort to identify the locus of the primary defect in this disease of electrolyte transport.

A tutorial in my laboratory would provide experience in membrane fractionation, ion transport studies, protein purification and resolution reconstitution.

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Dubinsky WP, Monti LB (1986) Solubilization and reconstitution of a Cl transporter from tracheal apical membrane. Am J Physiol 251:C713-C720.

Langridge-Smith JE, Dubinsky WP (1986) Relationship of the Donnan potential to the transmembrane Ph gradient in tracheal apical membrane vesicales. J Memb Biol 94:197-204.

Program Affiliation:
Program in Cell and Regulatory Biology (Physiology Track)