Samuel Kaplan, Ph.D.
1963, University of California, San Diego
The University of Texas Health Science Center at Houston
Medical School
Department of Microbiology and Molecular Genetics
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Samuel Kaplan, Ph.D. 1963, University of California, San Diego The University of Texas Health Science Center at Houston |
Research Interests:
The long-range objective is to understand how oxygen and light effect the molecular basis of gene control involved in the formation of the inducible macomolecular machine involved in energy generation in Rhodobacter sphaeroides. To accomplish these goals, we use a variety of genetic, physiologic, and biochemical approaches in studying gene structure, function and regulation, as well as membrane structure, synthesis and function. Such approaches include genome mapping, DNA sequencing, measurements of gene expression and the use of mutant organisms altered in gene structure and function which effect cellular control processes. The use of DNA array analysis allows for the study of newly discovered global regulators in both wild-type and mutant backgrounds. Physiologic experiments follow protein expression patterns in response to changing environmental states.
Selected Publications:
Seok J-S, Kaplan S, Oh J-I (2006) Interacting specificity of a histidine kinase and its cognate response regulator: the PrrBA system of Rhodobacter sphaeroides. Microbiol. 152:2479-2490. [abstract]
Kim YJ, Ko IJ, Lee JM, Kang HY, Kim YM, Kaplan S, Oh JI (2007) Dominant role of the cbb3 oxidase in regulation of photosynthesis gene expression through the PrrBA system in Rhodobacter sphaeroides 2.4.1. J. Bacteriol. 189(15):5617-5625. [abstract]
Mackenzie C, Eraso JM, Choudhary M, Roh JH, Zeng X, Bruscella P, Puskás Á, Kaplan S (2007) Postgenonic adventures with Rhodobacter sphaeroides Annu. Rev. Microbiol. 61:283-307. [abstract]
Pan D, Hu D, Liu R, Zeng X, Kaplan S, Lu HP (2007) Fluctuating two-state light harvesting in a photosynthetic membrane. J. Phys. Chem. 111:8948-8956. [abstract]
Hemp J, Han H, Roh JH, Kaplan S, Martinez TJ, Gennis RB (2007) Comparitive genomics and site-directed mutagenesis support the existence of only one input channel for protons in the C-family (cbb3 oxidase) of heme-copper oxygen reductases. Biochem. 46(35):9963-9972. [abstract]
Moskvin OV, Kaplan S, Gilles-Gonzalez M-A, Gomelsky M (2007) Novel heme-based oxygen sensor with a revealing evolutionary history J. Biol. Chem. 282(39):28740-28748. [abstract]
Zeng X, Roh JH, Callister SJ, Tavano CL, Donohue TJ, Lipton MS, Kaplan S (2007) Proteomic characterization of the Rhodobacter sphaeroides 2.4.1. photosynthetic membrane: identification of new proteins. J. Bacteriol. 189(20):7464-7474. [abstract]
Program Affiliation:
Program in Microbiology and Molecular Genetics