Charles F. Streckfus, D.D.S.
1978, University of Maryland School of Dental Surgery
UT-Houston Dental Branch
Diagnostic Sciences
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Charles F. Streckfus, D.D.S. 1978, University of Maryland School of Dental Surgery UT-Houston Dental Branch |
Research Interests: Cancer; aging; salivary function
The focus of the Tutorial Laboratory is to provide students with a basic understanding of the laboratory equipment and how it relates to both clinical and basic science research. Currently, there are four research laboratories located on the fourth floor of the UT Dental Branch which are dedicated to both clinical and basic science research. The students are first introduced to a review of laboratory ethics and regulatory compliance (e.g., IRB, OSHA, etc.). A brief overview of experimental design is also provided.
The students are then assigned a project which exposes them to the operation of any or all the following laboratory equipmentany: a Multiskan plate reader, PCR, BioPlex Suspension Array system, microplate auto washer, reciprocal water bath, speed vacuum, ultracentrifuge, pH meter, orbital shaker, pipettes, 4 ultra-low freezers with bar coded specimen identification system, fume hoods, RNA/DNA counter, equipment for performing 1 & 2d gels and Western blot procedures with a VersaDoc imaging system. The facilities also possess equipment to perform cellular studies. The laboratory is equipped with an inverted microscope, hood and cell incubator. The facility is also equipped with 2 Dell desktop computers that contain software for operating the laboratory equipment and for maintaining a laboratory quality control program. The laboratory is manned by two full time Sr. Research Associates. These laboratories also closely collaborate with the UT Medical School, Proteomics Division located on the fourth floor of the School of Medicine. The students will be further exposed to the operation of the LC-MS/MS mass spectrometer.
After their training, the students are allowed to complete the laboratory aspect of their assigned project. If the project is completed in a professional manner resulting in publishable information, the student will receive further exposure to data analyses and will write the methodology portion of her/his project. This report will be included in a manuscript and the student will be given co-authorship on the project.
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Streckfus CF, Bigler L (2005) The Use of soluble, salivary c-erbB-2 for the detection and post-operative follow-up of breast cancer in women: The results of a five year translational study. J Adv Dent Res, 18:17-22.
Streckfus C, Bigler L, Dellinger T, Kuhn M, Chouinard N, Dai X. The Expression of the c-erbB-2 Receptor Protein In Glandular Salivary Secretions. J Oral Path Med, 2004, 33:595-600.
Bigler LG, Streckfus CF (2004) A unique protein screening analysis of stimulated whole saliva from normal and breast cancer patients. Preclinica, 2(1): 52-56.
Brinkley J, Copeland L, Streckfus C, Tucci M, Benguzzi H (2003) Sustained delivery of HER-2/NEU antibody by TCPL delivery device using adult male rats as a model. Biomed Sci Inst., 39:324-328 .
Streckfus CF, Bigler LG, O’Bryan TM (2002) Effects of aging on salivary cytokine concentrations and whole saliva flow rates among women. Intl J Exp Clin Geron., 48(5):282-288.
Streckfus CF, Bigler L (2002) Saliva as a diagnostic fluid. (by invitation) Oral Diseases, 8(2):69-76.
Streckfus CF, Bigler L, Dellinger TD, Dai X, Kingman A, Thigpen JT (2000) The presence of c-erbB-2, and CA 15-3 in saliva and serum among women with breast carcinoma: a preliminary study. Clin Cancer Res., 6(6):2363-2370.
Streckfus CF, Bigler L, Tucci M, Thigpen JT (2000) The Presence of CA 15-3, c-erbB-2, EGFR, Cathepsin-D, and p53 in saliva among women with breast carcinoma. Cancer Invest., 18(2):101-109.
Patel M, Bigler S, Streckfus CF, Elkins S, Thigpen JT, Bigler L (2000) Expression of c-erbB-2 in node negative breast cancer patients. J Miss Acad Sci., 45(4):215-218.
Ghezzi EM, Wagner LA, Schork M, Metter EJ, Baum BJ, Streckfus CF, Ship JA (2000) Longitudinal influence of menopause and hormone replacement therapy on parotid flow rates in healthy women. J Geron Med Sci., 55A:m34-m42.
Streckfus CF, Bigler L, Dellinger TD, Pfeifer MR, Rose A, Thigpen JT (1999) CA 15-3, and c-erbB-2 presence in the saliva of Women. Clin Oral Invest., 3(3):138-143.
McIntyre R, Bigler L, Dellinger TD, Pfeifer MR, Mannery T, Streckfus CF (1999) Oral contraceptive use does not alter the expression of CA 15-3 and c-erbB-2 in the saliva of healthy women. Oral Surg Oral Med Oral Path., 88:687-690.